Streptavidin – HRP Conjugate

(Enzyme Horse Radish Peroxidase for the manufacture of Elisa Kits)

Description: conjugate of horseradish peroxidase (HRP) heteropolymer and streptavidin, 50% glycerol stock. Enzyme preparation for research use only.

Appearance                                                     clear, brownish, opalescent viscous liquid  

Concentration by biotin binding assay (ELISA)  1.1 mg/ml

Mean molecule radius by nefelometry               96 nm(SD 15%)

Reference ELISA test*:

sensitivity limit                                                    2,5 pg/ml

background noise                                             0.04 OD at 450/620 nm

working dilution                                                1:700-  1: 3000

Accelerated stability test**                               pass

(*)Two-step sandwich ELISA / 24 kDa protein analyte /sample volume 70 mkl / shaker incubation +37C /  TMB substrate /incubation time 40+10+15=65 min

(**) Remaining activity of the prediluted preparation in reference ELISA after 7 days incubation at +37C >80%